dpph free scavenging activity  (Nanjing Jiancheng Bioengineering Research Institute Co Ltd)

Journal: NPJ Biofilms and Microbiomes

Article Title: Human supplementation with Pediococcus acidilactici GR-1 decreases heavy metals levels through modifying the gut microbiota and metabolome

doi: 10.1038/s41522-022-00326-8

Figure Lengend Snippet: The OS status ( a ROS level, b GSH level, and c GSH-Px activity) and antioxidant activity ( d DPPH free radical – scavenging ability and e the total antioxidant capacity) of intestinal contents with different treatments. f The growth curve of GM in BGM growth medium containing 50 mg/L Cu under anaerobic condition. g PCA analysis of β-diversity in GM structures among the seven groups based on the Bray–Curtis distance. h The average relative abundances of GM at the class level. Data are expressed as means ± standard errors of the means in figure a – f . n = 3 per group in figure a – f and n = 5 per group in figure g and h . a – e statistical analysis was performed using one-way ANOVA with Tukey’s post hoc analysis (* p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001, ns represents not significant) using GraphPad Prism (version 8.0.1). Source data are provided as a source data file.

Article Snippet: Sampling was performed every 2 or 3 h, and the OD 600 value was measured. (2) Then, 1 mL of the supernatant was used to determine the DPPH free radical–scavenging activity and total antioxidant capacity of intestinal contents (Nanjing Jiancheng Institute of Biotechnology, Nanjing, China). (3) The precipitate of the fecal suspension was resuspended in 5 mL of PBS (pH = 7.0), and cell-free extract was obtained using the ultrasonic cell-break method (Biosafer 650-92) .

Techniques: Activity Assay, Antioxidant Activity Assay

Journal: LWT

Article Title: The first study of probiotic properties and biological activities of lactic acid bacteria isolated from Bat guano from Er-rachidia, Morocco

doi: 10.1016/j.lwt.2022.113224

Figure Lengend Snippet: Fig. 2. Antioxidant properties of LAB strains. A) DPPH free radical scavenging activity of cell-free supernatant (IC50 mg/mL). B) Total phenolic content of cell-free supernatant of the screened strains.

Article Snippet: DPPH free radical scavenging activity The 2,2-di-phényl-2-picrylhydrazyle (DPPH) radical scavenging activity was quantified according to (Hamad et al., 2020) with slight modifications.

Techniques: Activity Assay